University of Wisconsin–Madison
Prashant Sharma using the Olympus epifluorescence scope in the NIC.

NIC Epifluorescence

The NIC has a conventional upright epifluorescence microscope, the Olympus BX60. It features 4 cubes for DAPI (UV excitation), GFP (blue excitation) with long pass or short pass emission, and Rhodamine (green excitation). This microscope has 2.5x, 4x, 10x, 20x, 40x, and 60x objectives.

In addition to fluorescence, the NIC Olympus microscope has excellent brightfield optics including DIC. For special applications it is possible to use this instrument for dark field or polarizing microscopy.

The color camera is an Olympus DP 72, with 12.8 megapixels and 24 bit RGB. This camera is run by Olympus’ cellSens software.

Mouse heart tissue by Giangela Stokes
Section of diseased heart tissue from a young mouse. Image by Giangela Stokes, Ikeda Lab, Dept. of Medical Genetics.
five micrographs from NIC microscopes.