Our Zeiss Elyra PS.1 Combi system is primarily used for confocal microscopy (LSM 780). This instrument can also image samples using variable angle excitation and TIRF (Total Internal Reflection Fluorescence) microscopy.
Our Zeiss Elyra PS.1 Combi was installed in the NIC in 2014. It was made possible by a generous anonymous donation to the Department of Botany along with support from many units within UW-Madison including the College of Letters & Science, the College of Agriculture and Life Science, School of Medicine and Public Health, and the Graduate School along with contributions from a number of individual departments.
For confocal, six laser lines are available (405, 458, 488, 514, 561, and 633nm). It is also equipped with a 34 element internal GAsP detector (LSM 780) for single-molecule sensitive full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging.
Microscope optics include a 100x/1.46 alpha Plan-APO oil, the Plan-Apochromat 63x/1.40 oil, 40x/1.10 C-Apochromat for confocal in addition to 10x, 20x, and 63x (water) objectives for confocal imaging. Filter sets include a 4 laser line primary beamsplitter for optimal unmixing capability.
Additional capabilities are a joystick-controlled motorized stage, stage insert for temperature and CO2 control (Tokai Hit), and a fiber optic-coupled computer running Zeiss’ ZEN software.